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glass micropipette in situ  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company glass micropipette in situ
    Glass Micropipette In Situ, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 98/100, based on 3829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+micropipette+in+situ/Borosilicate+Glass/10__7554_slash_elife__15693-294-9-21
    Average 98 stars, based on 3829 article reviews
    glass micropipette in situ - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    In Situ:

    Article Title: Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adult Drosophila
    Article Snippet: .. In brief, individual GFP+ cells were picked with a glass micropipette in situ (pipettes were pulled from thick-walled borosilicate capillaries (BF120-69-10, Sutter Instruments) for initial tip opening of 1– 2 mm under a dissecting microscope and transferred into lysate buffer (0.9 PCR Reaction Buffer II, 1.35 mM MgCl2, 0.45% NP40, 4.5 mM DTT, 0.18 U/ml SUPERase-In, 0.36 U/ml RNase inhibitor, 12.5 nM UP1 primer, 0.045 mM dNTP mix) immediately, followed by reverse transcription using oligo(dT) primers to generated first-strand cDNA. ..

    Microscopy:

    Article Title: Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adult Drosophila
    Article Snippet: .. In brief, individual GFP+ cells were picked with a glass micropipette in situ (pipettes were pulled from thick-walled borosilicate capillaries (BF120-69-10, Sutter Instruments) for initial tip opening of 1– 2 mm under a dissecting microscope and transferred into lysate buffer (0.9 PCR Reaction Buffer II, 1.35 mM MgCl2, 0.45% NP40, 4.5 mM DTT, 0.18 U/ml SUPERase-In, 0.36 U/ml RNase inhibitor, 12.5 nM UP1 primer, 0.045 mM dNTP mix) immediately, followed by reverse transcription using oligo(dT) primers to generated first-strand cDNA. ..

    Polymerase Chain Reaction:

    Article Title: Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adult Drosophila
    Article Snippet: .. In brief, individual GFP+ cells were picked with a glass micropipette in situ (pipettes were pulled from thick-walled borosilicate capillaries (BF120-69-10, Sutter Instruments) for initial tip opening of 1– 2 mm under a dissecting microscope and transferred into lysate buffer (0.9 PCR Reaction Buffer II, 1.35 mM MgCl2, 0.45% NP40, 4.5 mM DTT, 0.18 U/ml SUPERase-In, 0.36 U/ml RNase inhibitor, 12.5 nM UP1 primer, 0.045 mM dNTP mix) immediately, followed by reverse transcription using oligo(dT) primers to generated first-strand cDNA. ..

    Reverse Transcription:

    Article Title: Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adult Drosophila
    Article Snippet: .. In brief, individual GFP+ cells were picked with a glass micropipette in situ (pipettes were pulled from thick-walled borosilicate capillaries (BF120-69-10, Sutter Instruments) for initial tip opening of 1– 2 mm under a dissecting microscope and transferred into lysate buffer (0.9 PCR Reaction Buffer II, 1.35 mM MgCl2, 0.45% NP40, 4.5 mM DTT, 0.18 U/ml SUPERase-In, 0.36 U/ml RNase inhibitor, 12.5 nM UP1 primer, 0.045 mM dNTP mix) immediately, followed by reverse transcription using oligo(dT) primers to generated first-strand cDNA. ..

    Generated:

    Article Title: Regulation of starvation-induced hyperactivity by insulin and glucagon signaling in adult Drosophila
    Article Snippet: .. In brief, individual GFP+ cells were picked with a glass micropipette in situ (pipettes were pulled from thick-walled borosilicate capillaries (BF120-69-10, Sutter Instruments) for initial tip opening of 1– 2 mm under a dissecting microscope and transferred into lysate buffer (0.9 PCR Reaction Buffer II, 1.35 mM MgCl2, 0.45% NP40, 4.5 mM DTT, 0.18 U/ml SUPERase-In, 0.36 U/ml RNase inhibitor, 12.5 nM UP1 primer, 0.045 mM dNTP mix) immediately, followed by reverse transcription using oligo(dT) primers to generated first-strand cDNA. ..



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    98
    Sutter Instrument Company glass micropipette in situ
    Glass Micropipette In Situ, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+micropipette+in+situ/Borosilicate+Glass/10__7554_slash_elife__15693-294-9-21
    Average 98 stars, based on 1 article reviews
    glass micropipette in situ - by Bioz Stars, 2026-11
    98/100 stars
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